Phylogenetic analysis of the “ECE” (CYC/TB1) clade reveals duplications predating the core eudicots

TitlePhylogenetic analysis of the "ECE" (CYC/TB1) clade reveals duplications predating the core eudicots
Publication TypeJournal Article
Year of Publication2006
AuthorsHowarth DG, Donoghue MJ
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Pagination9101-9106
Date Published2006 Jun 13
ISSN0027-8424
KeywordsAntirrhinum, Bayes Theorem, DNA-Binding Proteins, Evolution, Flowers, Gene Duplication, Molecular, Molecular Sequence Data, phylogeny, Plant Proteins, Transcription Factors
Abstract

Flower symmetry is of special interest in understanding angiosperm evolution and ecology. Evidence from the Antirrhineae (snapdragon and relatives) indicates that several TCP gene-family transcription factors, especially CYCLOIDEA (CYC) and DICHOTOMA (DICH), play a role in specifying dorsal identity in the corolla and androecium of monosymmetric (bilateral) flowers. Studies of rosid and asterid angiosperms suggest that orthologous TCP genes may be important in dorsal identity, but there has been no broad phylogenetic context to determine copy number or orthology. Here, we compare published data from rosids and asterids with newly collected data from ranunculids, caryophyllids, Saxifragales, and Asterales to ascertain the phylogenetic placement of major duplications in the “ECE” (CYC/TB1) clade of TCP transcription factors. Bayesian analyses indicate that there are three major copies of “CYC” in the ECE clade, and that duplications leading to these copies predate the core eudicots. CYC1 contains no subsequent duplications and may not be expressed in floral tissue. CYC3 exhibits similar patterns of duplication to CYC2 in several groups. Using RT-PCR, we show that, in flowers of Lonicera morrowii (Caprifoliaceae), DipsCYC2B is expressed in the four dorsal petals and not in the ventral petal. DipsCYC3B is expressed in flower and petal primordia, possibly most strongly in the ventral petal.

DOI10.1073/pnas.0602827103
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