@article {140,
	title = {Evolution of alcohol dehydrogenase genes in peonies (<i>Paeonia</i>): Phylogenetic relationships of putative nonhybrid species},
	journal = {Molecular Biology and Evolution},
	volume = {14},
	year = {1997},
	pages = {994 - 1007},
	abstract = {<p>Alcohol dehydrogenase genes were amplified by PCR, cloned, and sequenced from 11 putative nonhybrid species of the angiosperm genus Paeonia. Sequences of five exons and six intron regions of the Adh gene were used to reconstruct the phylogeny of these species. Two paralogous genes, Adh1A, and Adh2, were found; an additional gene, Adh1B, is also present in section Moutan. Phylogenetic analyses of exon sequences of the Adh genes of Paeonia and a variety of other angiosperms imply that duplication of Adh1 and Adh2 occurred prior to the divergence of Paeonia species and was followed by a duplication resulting in Adh1A and Adh1B. Concerted evolution appears to be absent between these paralogous loci. Phylogenetic analysis of only the Paeonia Adh exon sequences, positioning the root of the tree between the paralogous genes Adh1 and Adh2, suggests that the first evolutionary split within the genus occurred between the shrubby section Moutan and the other two herbaceous sections Oneapia and P</p>
},
	keywords = {Alcohol Dehydrogenase/*genetics, Cloning, Evolution, Genes, Genetic Variation, Molecular, Molecular*, Phylogeny*, Plant*, Plants/*enzymology, Plants/*genetics, Polymerase Chain Reaction, Species Specificity, Time},
	issn = {0737-4038},
	url = {http://search.ebscohost.com/login.aspx?direct=true\&db=cmedm\&AN=9335140\&site=eds-live},
	author = {Sang, Tao and Michael J Donoghue and Zhang, D L}
}
